Phenotyping of human apolipoprotein E from whole blood plasma by immunoblotting
نویسندگان
چکیده
منابع مشابه
Rapid apolipoprotein E phenotyping by immunofixation in agarose.
Conventional determination of apolipoprotein E isomorphs comprises ultracentrifugation of 1-5 ml serum, delipidation of very low density lipoproteins (VLDL), and isoelectric focusing (IEF) in polyacrylamide gels. In order to reduce the sample volume and to avoid nonspecific protein bands, immunoblotting was proposed. Now we describe a methodological variant that uses 25 microliters serum, repla...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1987
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)38599-0